7DXU

Crystal structure of the mk2h_deltaP peptide homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-22
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	54.476, 55.892, 56.423
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.191 - 2.314
R-factor	0.2576
Rwork	0.257
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dxr
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.300	2.300
Number of reflections	7930	1264
<I/σ(I)>	54.21
Completeness [%]	99.4
Redundancy	13.42
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2000 mM Ammonium sulfate, 100 mM CAPS/ Sodium hydroxide, pH 10.5, 200 mM Lithium sulfate