7DXR

Crystal structure of the mk2h peptide homodimer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-30
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	55.114, 56.031, 56.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.800 - 1.600
R-factor	0.2166
Rwork	0.214
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7du6
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	23802	3762
<I/σ(I)>	25.31
Completeness [%]	99.9
Redundancy	11.11
CC(1/2)	1.000	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1200mM Sodium phosphate monobasic/800mM Pottasium phosphate dibasic, 100 mM CAPS pH 10.5, 100mM Lithium sulfate