7DXP
Influenza H5N1 nucleoprotein in complex with nucleotides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.010, 60.630, 82.710 |
| Unit cell angles | 106.70, 109.01, 96.62 |
Refinement procedure
| Resolution | 24.501 - 2.300 |
| Rwork | 0.193 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.709 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.520 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.102 | 0.253 |
| Number of reflections | 37106 | 5475 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 90.4 | 91.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M MES, pH 6.0, 0.1 M NaCl and 11% PEG2000 |
