7DXM

Crystal structure of DltD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	81
Detector technology	PIXEL
Collection date	2020-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	58.273, 109.920, 282.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.960
Rwork	0.216
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bma
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.010
High resolution limit [Å]	2.960	2.960
Rmeas	0.215
Rpim	0.105	0.573
Number of reflections	38815	1904
<I/σ(I)>	10.85	1.5
Completeness [%]	99.2
Redundancy	4
CC(1/2)	0.984	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% glycerol, 14% [w/v] PEG6000, 170 mM (NH4)2SO4)