7DWN

Crystal structure of Vibrio fischeri DarR in complex with DNA reveals the transcriptional activation mechanism of LTTR family members

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	50.688, 77.456, 86.797
Unit cell angles	74.46, 78.16, 89.86

Refinement procedure

Resolution	25.235 - 2.320
R-factor	0.2301
Rwork	0.228
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ydw
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.820	48.820	2.390
High resolution limit [Å]	2.320	9.570	2.320
Rmerge	0.108	0.060	0.644
Rmeas	0.128	0.072	0.763
Rpim	0.068	0.039	0.404
Total number of observations	185387	2457	16083
Number of reflections	52682	749	4569
<I/σ(I)>	7.3	14.4	2.2
Completeness [%]	97.9	96.5	97.8
Redundancy	3.5	3.3	3.5
CC(1/2)	0.991	0.992	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Magnesium chloride 0.1M MES pH6.0 25% PEG3350