7DWD

Crystal structure of a glutathione S-transferase SbGSTU7 from Salix babylonica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	54.903, 54.903, 182.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.040 - 1.580
R-factor	0.2152
Rwork	0.215
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4top
RMSD bond length	0.011
RMSD bond angle	1.184
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.040	1.840
High resolution limit [Å]	1.580	1.580
Number of reflections	39294	3854
<I/σ(I)>	7.64
Completeness [%]	99.7
Redundancy	2
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289.15	HEPES sodium, PEG400, Ammonium sulfate