7DVG

Crystal structure of rice immune receptor RGA5-HMA5 mutant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9786
Spacegroup name	P 43 21 2
Unit cell lengths	65.995, 65.995, 132.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.040 - 2.800
R-factor	0.2013
Rwork	0.200
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zne
RMSD bond length	0.009
RMSD bond angle	1.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.100	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.110	0.840
Rmeas	0.110
Number of reflections	13666	736
<I/σ(I)>	13.2
Completeness [%]	99.5
Redundancy	12.1
CC(1/2)	0.990	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M ammonium nitrate, 20 % (w/v) PEG 3350