7DVC
Crystal structure of the computationally designed reDPBB_sym1 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-04-08 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.151, 98.151, 177.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.298 - 1.705 |
R-factor | 0.1658 |
Rwork | 0.166 |
R-free | 0.17710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Computationally Designed Mode |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 94555 | 14747 |
<I/σ(I)> | 33 | |
Completeness [%] | 99.6 | |
Redundancy | 28 | |
CC(1/2) | 1.000 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Sodium acetate pH3.8, 2M NaCl, 400 mM Lithium sulfate |