7DV3
Structure of Sulfolobus solfataricus SegA-AMPPNP protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-10-28 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.293, 62.058, 145.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.040 - 2.600 |
R-factor | 0.2184 |
Rwork | 0.213 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dut |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
Rmerge | 0.086 | 0.044 | 0.398 |
Rmeas | 0.099 | 0.051 | 0.457 |
Rpim | 0.048 | 0.025 | 0.219 |
Total number of observations | 59507 | ||
Number of reflections | 14837 | 1491 | 1469 |
<I/σ(I)> | 8.2 | ||
Completeness [%] | 95.5 | 88 | 97.2 |
Redundancy | 4 | 3.9 | 4.1 |
CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MES pH 6.0, Magnesium formate, Pentaerythritol ethoxylate (15/4 EO/OH) |