7DUS
Crystal structure of Mei2-RRM3 domain in S.pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 87.670, 87.670, 128.482 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.2178 |
| Rwork | 0.215 |
| R-free | 0.26320 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.125 | |
| Rmeas | 0.117 | |
| Rpim | 0.028 | 0.271 |
| Number of reflections | 10649 | 1034 |
| <I/σ(I)> | 21.879 | 2.16 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 16.5 | 14.3 |
| CC(1/2) | 0.933 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | 15% PEG 3350, 0.2MTri-Sodium Citrate Tris pH 8.6 |
