7DUI

Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with mRNA and cognate transfer RNA anticodon stem-loop and sisomicin derivative N1''PyrS bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9898
Spacegroup name	P 41 21 2
Unit cell lengths	400.000, 400.000, 173.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.726 - 3.620
R-factor	0.178
Rwork	0.177
R-free	0.21300
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (DEV-3318)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.726	2.980
High resolution limit [Å]	2.910	2.910
Rmerge	0.755	7.152
Number of reflections	293124	14334
<I/σ(I)>	9.65	0.4
Completeness [%]	96.5	64.3
Redundancy	123.1	67.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	17% MPD