7DU5

The structure of the M.tb MazF-mt1 toxin in complex with a fragment of cognate antitoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	83.271, 83.271, 141.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.640 - 2.650
R-factor	0.2554
Rwork	0.254
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kyt
RMSD bond length	0.006
RMSD bond angle	1.096
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.640	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.060	0.550
Number of reflections	8929	1158
<I/σ(I)>	30.5	6.5
Completeness [%]	99.8	99.6
Redundancy	18.8	20.1
CC(1/2)	0.999	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	20% PEG 3350, 0.1 M NaOAc pH 5.0, 0.1 M NaCl