7DU0

Structure of an type I-F anti-crispr protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	67.234, 34.929, 67.354
Unit cell angles	90.00, 118.87, 90.00

Refinement procedure

Resolution	29.910 - 1.960
R-factor	0.2536
Rwork	0.252
R-free	0.29520
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	1.960	1.960
Number of reflections	17975	1782
<I/σ(I)>	24.5
Completeness [%]	95.2
Redundancy	5
CC(1/2)	0.992	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 3350, sodium chloride