7DTJ

Crystal structure of the RecA2 domain of RNA helicase CGH-1 in C. elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	82.825, 82.825, 60.769
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.886 - 2.402
R-factor	0.2271
Rwork	0.226
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wax
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.886	2.488
High resolution limit [Å]	2.402	2.402
Rmerge	0.092	0.610
Number of reflections	9668	950
<I/σ(I)>	31.1	4.167
Completeness [%]	99.6
Redundancy	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Na acetate pH 5.0, 20% PEG 4000, 0.2M Ammonion acetate