7DTG

Crystal structure of lamin B1 Ig-like domain from human

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-08-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	144.647, 160.751, 132.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.830 - 3.600
R-factor	0.2258
Rwork	0.222
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3umn
RMSD bond length	0.002
RMSD bond angle	0.458
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Rmeas	0.080	0.360
Rpim	0.025	0.150
Number of reflections	17863	818
<I/σ(I)>	17.95	3.3
Completeness [%]	97.1	91.1
Redundancy	7.4	4.2
CC(1/2)	0.997	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	magnesium nitrate, PEG 3350