7DTF

Room temperature structure of lysozyme by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 43 21 2
Unit cell lengths	79.440, 79.440, 38.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.170 - 1.850
R-factor	0.2265
Rwork	0.224
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ig6
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.910
High resolution limit [Å]	1.850	1.850
Number of reflections	11022	1073
<I/σ(I)>	5.22	1.64
Completeness [%]	100.0	100
Redundancy	106.8	72.9
CC(1/2)	0.977	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, PEG 8000, NaCl