7DSR
Anthranilate phosphoribosyltransferase variant Gly141Asn from Saccharomyces cerevisiae in complex with 4-fluoroanthranilate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.209, 87.238, 109.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.360 - 2.500 |
| R-factor | 0.2059 |
| Rwork | 0.203 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dsm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.099 | 0.507 |
| Rpim | 0.027 | 0.145 |
| Number of reflections | 26794 | 1311 |
| <I/σ(I)> | 31.8 | 4.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.9 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | 20 % PEG 4000, 0.1 M Tris PH 8.0 |
