7DSJ
Anthranilate phosphoribosyltransferase from Saccharomyces cerevisiae in complex with PRPP and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.328, 89.488, 110.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.990 - 2.440 |
| R-factor | 0.1832 |
| Rwork | 0.180 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dsm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.491 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.440 | 2.450 |
| Rmeas | 0.097 | 0.484 |
| Rpim | 0.027 | 0.134 |
| Number of reflections | 29444 | 1440 |
| <I/σ(I)> | 24.3 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 287 | 0.1 M BIS-TRIS PH 6.5, 16% W/V PEG 10000 |
