7DSB

Crystal structure of actin capping protein in complex with V-1 and twinfilin C-terminal tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	90
Detector technology	CCD
Collection date	2020-11-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 21 21 21
Unit cell lengths	70.280, 87.480, 120.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.840 - 2.440
R-factor	0.2096
Rwork	0.207
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3aaa
RMSD bond length	0.003
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.840	45.840	2.590
High resolution limit [Å]	2.440	7.250	2.440
Rmerge	0.098	0.025	0.931
Rmeas	0.107	0.028	1.020
Number of reflections	28229	1189	4455
<I/σ(I)>	15.84	53.57	2.01
Completeness [%]	99.8	98.8	99.4
Redundancy	6.009	5.265	5.989
CC(1/2)	0.998	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% (w/v) PEG 3350, 5% (v/v) isopropanol, 0.1M HEPES-NaOH (pH = 7.5)