7DS6

Crystal structure of actin capping protein in complex with twinflin-1/CD2AP CPI chimera peptide (TWN-CDC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	90
Detector technology	CCD
Collection date	2020-11-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 21 21 21
Unit cell lengths	59.280, 64.610, 143.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.510 - 1.690
R-factor	0.2139
Rwork	0.212
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ds2
RMSD bond length	0.006
RMSD bond angle	0.822
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.070	48.070	1.800
High resolution limit [Å]	1.690	5.050	1.690
Rmerge	0.084	0.026	0.738
Rmeas	0.093	0.029	0.810
Number of reflections	54790	2532	9856
<I/σ(I)>	14.58	46.01	2.3
Completeness [%]	87.9	99.2	99.2
Redundancy	5.703	5.517	5.836
CC(1/2)	0.998	0.999	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	5% (w/v) PEG 3350, 5% (v/v) ethanol, 50mM Tris-HCl (pH = 7.0)