7DS2

Crystal structure of actin capping protein in complex with twinflin-1 C-terminus tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2012-12-26
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	52.040, 61.540, 161.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.940 - 1.950
R-factor	0.1675
Rwork	0.167
R-free	0.18350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3aa7
RMSD bond length	0.005
RMSD bond angle	0.737
Phasing software	MOLREP
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.970	48.970	2.070
High resolution limit [Å]	1.950	5.810	1.950
Rmerge	0.041	0.020	0.232
Rmeas	0.044	0.022	0.252
Number of reflections	37412	1604	5458
<I/σ(I)>	31.64	69.78	7.69
Completeness [%]	96.5	99.8	88.4
Redundancy	7.109	6.64	6.508
CC(1/2)	1.000	1.000	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	22.5% (w/v) PEG 400, 0.1M HEPES-NaOH (pH = 7.0)