7DS1
Crystal structure of Aspergillus oryzae Rib2 deaminase in complex with DARIPP (C-terminal deletion mutant at pH 6.5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.320, 90.778, 40.833 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.540 - 1.580 |
R-factor | 0.1839 |
Rwork | 0.183 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.540 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.045 | 0.622 |
Number of reflections | 31013 | 3056 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 100.0 | |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M MES pH 6.5, 0.2 M ammonium sulfate and 30% PEG 5000 MME |