7DS1

Crystal structure of Aspergillus oryzae Rib2 deaminase in complex with DARIPP (C-terminal deletion mutant at pH 6.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-01
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	59.320, 90.778, 40.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.540 - 1.580
R-factor	0.1839
Rwork	0.183
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.540	1.640
High resolution limit [Å]	1.580	1.580
Rmerge	0.045	0.622
Number of reflections	31013	3056
<I/σ(I)>	19.7
Completeness [%]	100.0
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.1 M MES pH 6.5, 0.2 M ammonium sulfate and 30% PEG 5000 MME