7DRZ

Crystal structure of Aspergillus oryzae Rib2 deaminase (C-terminal deletion mutant) at pH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-12
Detector	RAYONIX MX300HE
Wavelength(s)	0.9762
Spacegroup name	P 41 21 2
Unit cell lengths	87.093, 87.093, 61.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.120 - 1.700
R-factor	0.1961
Rwork	0.195
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.100	0.780
Number of reflections	26416	2577
<I/σ(I)>	21.7
Completeness [%]	100.0
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	2 M sodium chloride and 0.1 M sodium acetate pH 4.6