7DRY

Crystal structure of Aspergillus oryzae Rib2 deaminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-05
Detector	RAYONIX MX300HE
Wavelength(s)	0.9762
Spacegroup name	P 41 21 2
Unit cell lengths	87.410, 87.410, 60.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.850 - 1.440
R-factor	0.1728
Rwork	0.172
R-free	0.19790
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.490
High resolution limit [Å]	1.440	1.440
Rmerge	0.042	0.746
Number of reflections	43068	4217
<I/σ(I)>	33.8
Completeness [%]	99.9
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		288	1.5 M ammonium sulfate and 0.1 M sodium acetate pH 4.6