7DRQ
Crystal structure of polysaccharide lyase Uly1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.993, 99.998, 95.304 |
| Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
| Resolution | 37.110 - 1.790 |
| R-factor | 0.1585 |
| Rwork | 0.157 |
| R-free | 0.18240 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.110 | 1.860 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.118 | 0.286 |
| Rmeas | 0.128 | 0.309 |
| Rpim | 0.049 | 0.116 |
| Number of reflections | 101227 | 9595 |
| <I/σ(I)> | 22.79 | |
| Completeness [%] | 99.2 | 94.48 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM calcium acetate, 100 mM MES (pH 6.0), 20% (w/v) PEG 8000 |
