7DRG

Pseudomonas aeruginosa T6SS protein PA0821

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	81.244, 46.036, 97.740
Unit cell angles	90.00, 95.70, 90.00

Refinement procedure

Resolution	26.950 - 1.600
R-factor	0.1841
Rwork	0.183
R-free	0.20670
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.656
High resolution limit [Å]	1.599	1.599
Number of reflections	94899	8949
<I/σ(I)>	38.035
Completeness [%]	99.3
Redundancy	6.6
CC(1/2)		0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.4 M Sodium acetate trihydrate and 0.1M Sodium cacodylate trihydrate pH 6.4