7DRB

Crystal structure of plant receptor like protein RXEG1 with xyloglucanase XEG1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-10-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9790
Spacegroup name	P 1 21 1
Unit cell lengths	103.888, 92.844, 154.526
Unit cell angles	90.00, 107.91, 90.00

Refinement procedure

Resolution	49.010 - 3.300
R-factor	0.2304
Rwork	0.227
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mna
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	41355	4059
<I/σ(I)>	13
Completeness [%]	97.3
Redundancy	3.6
CC(1/2)	0.712	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1M MES monohydrate pH 6.0, 14% (w/v) polyethylene glycol (PEG) 4000