7DPX

Crystal structure of the SRCR domain of human SCARA1/CD204

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-10-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	37.031, 57.086, 62.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.795 - 2.002
R-factor	0.2119
Rwork	0.208
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j02
RMSD bond length	0.006
RMSD bond angle	0.779
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rpim	0.027	0.171
Number of reflections	9366
<I/σ(I)>	18.3
Completeness [%]	96.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1M HEPES pH7.5, 20% PEG 8000, calcium