7DPE

RNA methyltransferase METTL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97776
Spacegroup name	C 1 2 1
Unit cell lengths	90.655, 93.494, 70.885
Unit cell angles	90.00, 107.19, 90.00

Refinement procedure

Resolution	29.324 - 2.751
R-factor	0.213
Rwork	0.210
R-free	0.26160
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (v1.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.850
High resolution limit [Å]	2.750	5.910	2.750
Rmerge	0.074	0.058	0.317
Rmeas	0.081	0.063	0.363
Rpim	0.032	0.024	0.173
Total number of observations	81664
Number of reflections	14645	1501	1438
<I/σ(I)>	5
Completeness [%]	99.4	100	97.2
Redundancy	5.6	6.6	3.8
CC(1/2)		0.998	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M sodium malonate pH5.0 and 12% PEG3350