7DOL

Mycoplasma genitalium RNase R in complex with double-stranded RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	75.414, 96.085, 117.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.294 - 2.002
R-factor	0.221
Rwork	0.220
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xgu
RMSD bond length	0.008
RMSD bond angle	1.156
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2148)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.119	1.087
Number of reflections	57987	2865
<I/σ(I)>	17.9	1.5
Completeness [%]	99.9
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	100 mM CHES (pH9.5), 20% PEG 8000