7DMX

Photocleavable Fluorescent Protein in green form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	60.360, 112.760, 144.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.390 - 2.100
R-factor	0.2075
Rwork	0.205
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hqk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.390	2.180
High resolution limit [Å]	2.100	2.100
Rmerge		0.709
Number of reflections	57043	9153
<I/σ(I)>	9.73
Completeness [%]	96.8
Redundancy	3.4
CC(1/2)	0.997	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MIB buffer pH 6.0, 25% w/v PEG 1500