7DMW
Crystal structure of CcpC regulatory domain in complex with citrate from Bacillus amyloliquefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9826 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.963, 90.898, 105.531 |
Unit cell angles | 90.00, 106.18, 90.00 |
Refinement procedure
Resolution | 29.710 - 2.290 |
R-factor | 0.2096 |
Rwork | 0.207 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.946 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.290 | 2.300 |
Number of reflections | 55746 | 2427 |
<I/σ(I)> | 36.2 | 2.64 |
Completeness [%] | 96.5 | 84.1 |
Redundancy | 5.3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | 10% PEG 6000 5% MPD 0.1 M HEPES (pH7.5) |