7DM5

The structure of the Arabidopsis thaliana guanosine deaminase in reaction with guanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-05-12
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	118.566, 118.566, 39.405
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.560 - 2.200
R-factor	0.17
Rwork	0.167
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.003
RMSD bond angle	0.548
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.560	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.110	0.320
Number of reflections	16363	2364
<I/σ(I)>	15.1	5.9
Completeness [%]	99.9	100
Redundancy	6.9	7.1
CC(1/2)	0.996	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)