7DM0

Biofilm associated protein - C region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	49.354, 45.899, 74.391
Unit cell angles	90.00, 91.92, 90.00

Refinement procedure

Resolution	41.750 - 1.550
R-factor	0.199
Rwork	0.198
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m3a
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.750	1.605
High resolution limit [Å]	1.550	1.550
Number of reflections	47661	4084
<I/σ(I)>	12.43
Completeness [%]	97.8
Redundancy	4.1
CC(1/2)	0.997	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1 M Tris hydrochloride pH8.5, 0.1M Magnesium chloride, 20% PEG400, 20% PEG8000