7DLY

Crystal structure of Arabidopsis ACS7 mutant in complex with PPG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	H 3
Unit cell lengths	164.425, 164.425, 97.881
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.160 - 2.940
R-factor	0.2292
Rwork	0.224
R-free	0.27740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dlw
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.940	2.950
Rmerge	0.086
Number of reflections	20168	2093
<I/σ(I)>	11.2
Completeness [%]	96.3
Redundancy	5.4
CC(1/2)		0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	293	0.1 M HEPES pH 7.6, 6% (w/v) PEG 10000