7DLR
Mycobacterium tuberculosis enolase mutant - E163A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-08-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 140.198, 140.198, 90.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.710 - 2.250 |
| R-factor | 0.1637 |
| Rwork | 0.161 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cll |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.831 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.071 | 0.563 |
| Number of reflections | 21190 | 991 |
| <I/σ(I)> | 20.37 | 2.16 |
| Completeness [%] | 98.3 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3350, 0.2 M Ammonium acetate, Bis Tris pH 6.5 |
