7DLN

Structure of lysozyme obtained on SSRF using serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	79.000, 79.000, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.244 - 2.150
R-factor	0.1887
Rwork	0.187
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.009
RMSD bond angle	1.149
Data reduction software	CrystFEL (0.8.0)
Data scaling software	CrystFEL (0.8.0)
Phasing software	PHENIX (1.11.1)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.250	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	6887	659
<I/σ(I)>	3.98	2.45
Completeness [%]	99.3
Redundancy	14.17
CC(1/2)	0.885	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	sodium chloride, PEG20000