7DLD
Crystal structures of (S)-carbonyl reductases from Candida parapsilosis in different oligomerization states
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.262, 114.380, 126.371 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.220 - 1.750 |
R-factor | 0.192 |
Rwork | 0.188 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ctm |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.220 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 50791 | 4991 |
<I/σ(I)> | 16.7 | |
Completeness [%] | 99.7 | |
Redundancy | 13.2 | |
CC(1/2) | 0.999 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.3 | 293.15 | 200 mM Ammonium Chloride, 20% PEG 3350, pH 6.3 |