7DLB

Crassostrea gigas ferritin mutant-D119K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2020-11-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9375
Spacegroup name	I 2 3
Unit cell lengths	150.702, 150.702, 150.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.555 - 2.502
R-factor	0.1976
Rwork	0.195
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fha
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	PHENIX (1.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.555	2.592
High resolution limit [Å]	2.500	2.500
Number of reflections	19777	1972
<I/σ(I)>	5.1
Completeness [%]	100.0
Redundancy	9.5
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	MPD, Tris base/ Hydrochloric acid, Sodium chloride