7DL8

Crystal structure of ALBA1 from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	C 2 2 21
Unit cell lengths	70.028, 86.236, 211.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.118 - 2.459
R-factor	0.2399
Rwork	0.238
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	rosetta model
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.459	2.460
Rmerge	0.093	0.830
Number of reflections	23706	2342
<I/σ(I)>	18.9	3.6
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.999	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Potassium sodium tartrate tetrahydrate, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 2.0M Ammonium sulfate