7DL5
Crystal structure of Thermotoga Maritima ferritin mutant at 2.3 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.093, 107.093, 110.589 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.610 - 2.300 |
| R-factor | 0.1824 |
| Rwork | 0.178 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vlg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.768 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.610 | 2.384 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 20913 | 2059 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.2 | 0.1 M MES pH 6.5 1.6M Magnesium Sulfate Hexahydrate |
