7DKI

Silk worm FKBP, isoform-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2019-01-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 32
Unit cell lengths	52.409, 52.409, 77.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.388 - 2.000
R-factor	0.1592
Rwork	0.155
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fkb
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.099	0.050	0.445
Rmeas	0.111	0.056	0.531
Rpim	0.050	0.025	0.286
Total number of observations	74774
Number of reflections	16023	811	802
<I/σ(I)>	4
Completeness [%]	100.0	100	99.8
Redundancy	4.7	5.2	3.2
CC(1/2)		0.998	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	1M sodium citrate tribasic dihydrate, 0.1M sodium cacodylate pH 6.5