7DKB
Stenotrophomonas maltophilia DPP7 in complex with Val-Tyr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-10-02 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.490, 73.330, 151.820 |
| Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.030 |
| Rwork | 0.201 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wol |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.576 |
| Data reduction software | xia2 (0.5.161) |
| Data scaling software | XDS |
| Phasing software | PHASER (2.6) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.256 | 1.487 |
| Rmeas | 0.275 | |
| Rpim | 0.100 | 0.576 |
| Number of reflections | 100498 | 3191 |
| <I/σ(I)> | 7.9 | 1.4 |
| Completeness [%] | 93.7 | 60.1 |
| Redundancy | 7.6 | 7.5 |
| CC(1/2) | 0.397 | 0.397 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M Amm Acetate, 20%w/v PEG 8000 |
