7DJY
Clec4f Nanobody 322
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 42 |
| Unit cell lengths | 80.469, 80.469, 107.733 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.800 - 2.700 |
| R-factor | 0.23202 |
| Rwork | 0.228 |
| R-free | 0.26598 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5imm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.258 | |
| Number of reflections | 18555 | 1855 |
| <I/σ(I)> | 5.74 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.981 | 0.533 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M Citric acid, 0.04M BIS-TRIS propane pH 5.0, 16% polyethylene glycol 3350 |
