7DJX
Clec4f Nanobody 246
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.535, 73.535, 94.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.630 - 1.980 |
| R-factor | 0.20131 |
| Rwork | 0.200 |
| R-free | 0.21649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5imm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.393 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.630 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.077 | 1.569 |
| Number of reflections | 18661 | 1776 |
| <I/σ(I)> | 23 | |
| Completeness [%] | 99.7 | |
| Redundancy | 24 | |
| CC(1/2) | 0.998 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02M Magnesium chloride hexahydrate, 0.1M HEPES, pH 7.5, 22% poly acrylic acid sodium salt 5100 |
