7DJJ

Structure of four truncated and mutated forms of quenching protein lumenal domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-07-13
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97861
Spacegroup name	P 43 21 2
Unit cell lengths	80.540, 80.540, 172.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.520 - 2.698
R-factor	0.195938430209
Rwork	0.194
R-free	0.23374
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.323
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.698	2.698
Rpim	0.031
Number of reflections	16265	792
<I/σ(I)>	24.75
Completeness [%]	100.0	100
Redundancy	12.5
CC(1/2)		0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	291.15	1.2 M NaH2PO4, 0.8 MK2HPO4, 0.2 M Li2SO4. 0.1 M Capso, pH 9.5