7DI0

Crystal structure of the rationally designed apDPBB_sym_79 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-11
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	30.254, 77.875, 108.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.630 - 1.600
R-factor	0.1805
Rwork	0.179
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dg7
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	65486	10554
<I/σ(I)>	10.08
Completeness [%]	99.7
Redundancy	3.84
CC(1/2)	0.997	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	100mM Tris-HCl, pH 8.5, 25% PEG 3350, 200mM Lithium sulfate