7DHB

mutant V507M coiled coil domain of Trypanosoma brucei coronin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	273
Detector technology	PIXEL
Collection date	2019-12-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	I 4 2 2
Unit cell lengths	90.760, 90.760, 83.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.520 - 3.020
R-factor	0.216
Rwork	0.214
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cx2
RMSD bond length	0.002
RMSD bond angle	0.448
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHASES
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.520	3.128
High resolution limit [Å]	3.020	3.020
Number of reflections	3325	345
<I/σ(I)>	2.46
Completeness [%]	90.7
Redundancy	1.8
CC(1/2)	0.998	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	300	3 M NaCl, 0.1m HEPESE 7.5