7DH6
Crystal structure of PLRG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.005, 82.124, 99.388 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 38.156 - 2.584 |
| R-factor | 0.2287 |
| Rwork | 0.226 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yvd |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.063 | 0.804 |
| Number of reflections | 33636 | 3125 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 Calcium chloride 20% PEG 3350 |
