7DH4

Double mutant- V493I-V507M coiled coil domain of Trypanosoma brucei coronin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	200
Detector technology	PIXEL
Collection date	2019-06-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	I 4 2 2
Unit cell lengths	91.230, 91.230, 83.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.620 - 2.410
R-factor	0.238
Rwork	0.237
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dgx
RMSD bond length	0.002
RMSD bond angle	0.408
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.610	2.496
High resolution limit [Å]	2.410	2.410
Number of reflections	6754	681
<I/σ(I)>	6.57
Completeness [%]	95.1
Redundancy	2
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	300	3 M NaCl and HEPESE 7.5